- Complexity of Neurological Diseases: Neurological disorders often involve multiple pathways and mechanisms, making it difficult to identify single biomarkers. - Standardization: There is a need for standardized protocols to ensure consistency in biomarker measurement across different laboratories. - Ethical Concerns: The use of genetic biomarkers raises ethical questions about privacy and the potential for genetic discrimination. - Cost: Developing and validating biomarkers is an expensive process, which can limit their availability and implementation in clinical practice.