The procedure is typically performed in a hospital or clinic setting. Here is a step-by-step overview:
1. Preparation: The patient will be asked to lie on their side or sit up and lean forward to curve the spine. 2. Anesthesia: A local anesthetic is applied to numb the area where the needle will be inserted. 3. Insertion: A thin, hollow needle is carefully inserted into the lower part of the spine, usually between the third and fourth lumbar vertebrae. 4. Collection: Once the needle is in place, CSF is collected in sterile tubes for analysis. 5. Completion: The needle is removed, and a bandage is applied to the puncture site.